• Complete mitochondrial genome sequences of the Arctic Ocean codfishes Arctogadus glacialis and Boreogadus saida reveal oriL and tRNA gene duplications 

      Breines, Ragna; Ursvik, Anita; Nymark, Marianne; Johansen, Steinar; Coucheron, Dag-Hugo (Journal article; Tidsskriftartikkel; Peer reviewed, 2008-05-27)
      We have determined the complete mitochondrial genome sequences of the codWshes Arctogadus glacialis and Boreogadus saida (Order Gadiformes, Family Gadidae). The 16,644 bp and 16,745 bp mtDNAs, respectively, contain the same set of 37 structural genes found in all vertebrates analyzed so far. The gene organization is conserved compared to other Gadidae species, but with one notable exception. ...
    • Datasenter i Salangen. 

      Johansen, Steinar; Johnsen, Ola Marius; Schjefstad, Trond (Others; Andre, 1983)
      Dette er en rapport som beskriver planer for et data/kommunikasjonssenter i Salangen kommune.
    • Differential expression patterns of conserved miRNAs and isomiRs during Atlantic halibut development 

      Bizuayehu, Teshome; Lanes, Carlos Fredrico Ceccon; Furmanek, Tomasz; Karlsen, Bård Ove; Fernandes, Jorge; Johansen, Steinar; Babiak, Igor (Journal article; Tidsskriftartikkel; Peer reviewed, 2012)
      MicroRNAs (miRNAs) play a major role in animal ontogenesis. Size variants of miRNAs, isomiRs, are observed along with the main miRNA types, but their origin and possible biological role are uncovered yet. Developmental profiles of miRNAs have been reported in few fish species only and, to our knowledge, differential expressions of isomiRs have not yet been shown during fish development. Atlantic ...
    • Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemones 

      Urbarova, Ilona; Karlsen, Bård Ove; Okkenhaug, Siri; Seternes, Ole Morten; Johansen, Steinar; Emblem, Åse (Journal article; Tidsskriftartikkel; Peer reviewed, 2012-10-18)
      Marine bioprospecting is the search for new marine bioactive compounds and large-scale screening in extracts represents the traditional approach. Here, we report an alternative complementary protocol, called digital marine bioprospecting, based on deep sequencing of transcriptomes. We sequenced the transcriptomes from the adult polyp stage of two cold-water sea anemones, Bolocera tuediae and Hormathia ...
    • Giant group I intron in a mitochondrial genome is removed by RNA back-splicing 

      Chi, Sylvia Ighem; Dahl, Mikael; Emblem, Åse; Johansen, Steinar (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-06-01)
      <p><i>Background - </i>The mitochondrial genomes of mushroom corals (Corallimorpharia) are remarkable for harboring two complex group I introns; ND5-717 and COI-884. How these autocatalytic RNA elements interfere with mitochondrial RNA processing is currently not known. Here, we report experimental support for unconventional processing events of ND5-717 containing RNA. <p><i>Results - </i>We obtained ...
    • Nuclear group I introns in self-splicing and beyond 

      Hedberg, Annica; Johansen, Steinar (Journal article; Tidsskriftartikkel; Peer reviewed, 2013)
      Group I introns are a distinct class of RNA self-splicing introns with an ancient origin. All known group I introns present in eukaryote nuclei interrupt functional ribosomal RNA genes located in ribosomal DNA loci. The discovery of the Tetrahymena intron more than 30 years ago has been essential to our understanding of group I intron catalysis, higher-order RNA structure, and RNA folding, but ...
    • Performance Comparison of Digital microRNA Profiling Technologies Applied on Human Breast Cancer Cell Lines 

      Knutsen, Erik; Fiskaa, Tonje; Ursvik, Anita; Jørgensen, Tor Erik; Perander, maria; Lund, Eiliv; Seternes, Ole Morten; Johansen, Steinar (Journal article; Tidsskriftartikkel; Peer reviewed, 2013)
      MicroRNA profiling represents an important first-step in deducting individual RNA-based regulatory function in a cell, tissue, or at a specific developmental stage. Currently there are several different platforms to choose from in order to make the initial miRNA profiles. In this study we investigate recently developed digital microRNA high-throughput technologies. Four different platforms were ...
    • Polyphyletic origin of the genus Physarum (Physarales, Myxomycetes) revealed by nuclear rDNA mini-chromosomal analysis and group I intron synapomorphy 

      Nandipati, Satish C R; Haugli, Kari B.; Coucheron, Dag-Hugo; Haskins, Edward F.; Johansen, Steinar (Journal article; Tidsskriftartikkel; Peer reviewed, 2012-08-31)
      Physarales represents the largest taxonomic order among the plasmodial slime molds (myxomycetes). Physarales is of particular interest since the two best-studied myxomycete species, Physarum polycephalum and Didymium iridis, belong to this order and are currently subjected to whole genome and transcriptome analyses. Here we report molecular phylogeny based on ribosomal DNA (rDNA) sequences that ...
    • Site-specific reverse splicing of a HEG-containing group I intron in ribosomal RNA 

      Birgisdottir, Åsa Birna; Johansen, Steinar (Journal article; Tidsskriftartikkel; Peer reviewed, 2005)
      The myxomycete Didymium iridis (isolate Panama 2) contains a mobile group I intron named Dir.S956-1 after position 956 in the nuclear small subunit (SSU) rRNA gene. The intron is efficiently spread through homing by the intron-encoded homing endonuclease I-DirI. Homing endonuclease genes (HEGs) usually spread with their associated introns as a unit, but infrequently also spread independent ...